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Ação de Micoflora de efluentes agroindustriais sobre diferentes corantes e substratos lignocelulósicos - doi: 10.4025/actascibiolsci.v33i4.6187 Biological Sciences
Perovano Filho, Natalino; UNIVERSIDADE FEDERAL DE ALAGOAS, INSTITUTO DE QUÍMICA E BIOTECNOLOGIA; Silva, Kelly Fernanda Seára da; UNIVERSIDADE FEDERAL DE ALAGOAS, INSTITUTO DE QUÍMICA E BIOTECNOLOGIA; López, Ana Maria Queijeiro; UNIVERSIDADE FEDERAL DE ALAGOAS, INSTITUTO DE QUÍMICA E BIOTECNOLOGIA.
Enzimas extracelulares de fungos são importantes para sua sobrevivência em ambientes inóspitos, sendo capazes de degradar compostos recalcitrantes. Fungos filamentosos secretam hidrolases e fenoloxidases capazes de degradar complexos de estruturas aromáticas, como de alguns xenobióticos. O propósito deste trabalho foi testar diferentes corantes (Azul de Metileno, Azure B, Verde Malaquita e Cristal Violeta), em substituição ao composto Poly R478, no método de seleção de microrganismos degradadores de fenóis em meio sólido, além de comparar tal atividade de Micoflora isolada de efluentes de indústria sucroalcooleira com a de outros fungos adquiridos em coleção oficial. Os corantes testados revelaram-se ótimos substitutos do Poly R478. O isolado que...
Tipo: Por composição bioquímica; Palavras-chave: 2.12.02.00-1 degradação biológica; Fenoloxidases; Bagaço de cana; Serragem de madeira; Azul de Metileno; Cladosporium Microbiologia Aplicada biological degradation; Phenol; Sugarcane bagasse; Sawing wood; Methylene blue; Cladosporium.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6187
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Analysis of the Phenol and Flavonoid Content from Basil Leaves (Ocimum Americanum L) Extract Using Pulsed Electric Field (PEF) Pre-Treatment CIGR Journal
Hendrawan, Yusuf; Sabrinauly, Sabrinauly; Hawa, La Choviya; Argo, Bambang Dwi; Rachmawati, Muchnuria.
Basil leaves (Ocimum Americanum Linn) are commonly known to contain phenol and flavonoid compounds which can act as antimicrobial and antioxidants. In general, the bioactive compound can be obtained by conventional extraction method, maceration. To increase the amount of bioactive compounds which can be extracted, it is necessary to conduct pre-treatment using Pulsed Electric Field (PEF). PEF has an advantage in increasing the compound transfer contained in cells to be extracted from the plant tissues via electroporation mechanisms, without causing significant temperature changes. This study aims to determine the effect of electric field strength variations (2, 3, and 4 kV/cm) and duration of pre-treatment (1, 2, and 3 minutes) to total phenol and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural Engineering Basil leaves; Phenol; Flavonoid; Pulsed electric field.
Ano: 2019 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/5240
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Effect of Chemical-Hydrothermal Pretreatments on Compositional and Morphological Changes of Spruce Wood Exploited in Biogas Systems CIGR Journal
Rahimi-Ajdadi, Fatemeh; Esmaili, Masoomeh.
Agricultural crops and forest residues are of valuable resources to produce biofuel due to anaerobic digestion. But, the recalcitrance nature of these lignocellulose residues limits its enzymatic degradation. Therefore, efficient pretreatments prior to anaerobic digestion are essential. Hydrothermal-chemical pretreatments using Phenol (Ph), Sodium Hydroxide (SH), and Phosphoric Acid (PA), and combined pretreatments included Ph+SH and Ph+PA as chemical pretreatments were used for spruce wood. The samples were put into the autoclave at 134º C for 20 min. Acid hydrolysis, FTIR and SEM analyses were carried out. The results indicated that all pretreatments were effective lignin removal having the highest value for Ph (42.362%). Adding Ph to PA increased lignin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofuel; Combined pretreatment; Lignin removal; Phenol; SEM analysis.
Ano: 2021 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/6855
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Interaction of Olive Oil Phenol Antioxidant Components with Low-density Lipoprotein Biol. Res.
LAMUELA-RAVENTÓS,ROSA M; GIMENO,EVA; FITÓ,MONTSE; CASTELLOTE,ANA-ISABEL; COVAS,MARIBEL; DE LA TORRE-BORONAT,M. CARMEN; LÓPEZ-SABATER,M. CARMEN.
Phenolic compounds have shown to inhibit LDL oxidation in vitro and ex vivo; however, they are hydrosoluble compounds while LDL is a lipoprotein. Analysis of phenolic compounds in LDLs by HPLC is necessary to demonstrate their binding capacity to lipoproteins. We developed and validated a solid phase extraction method (SPE) that allowed us the purification of LDL samples and their analysis by HPLC. This methodology allowed us to demonstrate the in vitro binding capacity of tyrosol, one of the main phenolic compounds in olive oil, to LDL. In the intervention dietary study with volunteers, food rich in phenolic compounds affected LDL composition. Changes in LDL phenolics composition are not observed after the short-term ingestion of food rich in phenolic...
Tipo: Journal article Palavras-chave: Antioxidant; LDL; Olive oil; Oxidation; Phenol; Tyrosol.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200011
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Método não-invasivo na obtenção de DNA de búfalos - DOI: 10.4025/actascianimsci.v30i4.4839 Animal Sciences
Ribeiro, Juliana Maria; UEM; Gasparino, Eliane; UEM; Marques, Débora Sommer; UEM; Luizetti, Fabiane; UEM; Soares, Maria Amélia Menck; UEM; Zeoula, Lúcia Maria; UEM.
O objetivo do trabalho foi comparar dois diferentes protocolos de extração de DNA de pelos de búfalos (Bubalus bubalis) e comparar três regiões de coleta de material (nuca, paleta direita e testa). Foram utilizados quatro búfalos com três repetições por animal e por região. No protocolo 1, foi utilizada a técnica do fenol-clorofórmio e no protocolo 2, a técnica de extração com CTAB. O protocolo 2 apresentou maior média de concentração de DNA para as amostras de pelos. Em relação ao local de retirada dos pelos, não foram encontradas diferenças significativas, porém nota-se que a região da testa dos animais apresentou maior concentração de DNA quando extraído com CTAB. Com relação à praticidade de utilização dos dois métodos avaliados, o protocolo 2, além de...
Tipo: Análise laboratorial Palavras-chave: 5.05.04.00-2 Reprodução Animal CTAB; Extração de DNA; Fenol; Pelos. Genética e Melhoramento dos Animais Domésticos CTAB; DNA extraction; Phenol; Fur..
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4839
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Physiological changes of Candida tropicalis population degrading phenol in fed batch reactor BABT
Komarkova,Eliska; Paca,Jan; Klapkova,Eva; Stiborova,Marie; Soccol,Carlos R; Sobotka,Miroslav.
Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be formed. An accumulation of these products inhibited the cell growth under aerobic conditions. Another impact was a decrease of the phenol hydroxylase activity as the key enzyme of the phenol degradation pathway at the end of the cell respiration activity. This decrease is linked with the above mentioned product inhibition. The cell death studied by fluorescent probe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenol; Biodegradation; Candida tropicalis; Phenol hydroxylase; Starvation stress; Viability.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400007
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Respirometry kinetics of phenol oxidation by Comamonas testosteroni Pb50 under various conditions of nutritional stress BABT
Paca,Jan; Kosteckova,Alena; Pacova,Leona; Prell,Ales; Halecky,Martin; Paca Jr.,Jan; Stiborova,Marie; Kozliak,Evguenii; Soccol,Carlos Ricardo.
Kinetics of phenol biodegradation using suspended biomass of Comamonas testosteroni Pb50 (monoculture) was measured under conditions of nutrient abundance, limitation, and prolonged cell starvation in a fed-batch reactor, with phenol being the sole carbon and energy source. The pre-washed cells were applied for measurement of the phenol and oxygen uptake rates at varied starting phenol concentrations with the kinetic parameters calculated using the Haldane model. The results revealed that nutrient limitation significantly suppressed the maximum value of exogenous respiration rate while the endogenous respiration rate, affinity and tolerance to phenol increased. By contrast, cell starvation resulted in a drop of both the exogenous and endogenous respiration...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Respirometry; Kinetics; Phenol; Nutrition stress.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600030
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Seasonal variation of phenolic content in galled and non-galled tissues of Calliandra brevipes Benth (Fabaceae: Mimosoidae) Acta Botanica
Detoni,Michelle de Lima; Vasconcelos,Eveline Gomes; Rust,Naiara Miranda; Isaias,Rosy Mary dos Santos; Soares,Geraldo Luiz Gonçalves.
Two species, Tanaostigmodes ringueleti and T. mecanga, induce distinct galls on Calliandra brevipes Benth (Fabaceae: Mimosoidae), a globose and a fusiform gall morphotype. Seasonal changes of phenolic content in the tissues of the two distinct galls were compared to those of non-galled leaves and stems of the host plants over one year. The variation in the phenolic content profiles was similar in both non-galled and galled tissues, and was primarily associated with changes in the levels of rainfall, indicating a direct response to hydric stress. In periods of drastic changes in water precipitation, the alterations were significantly higher in non-galled than in galled tissues suggesting that the gall inducers might limit the variation in the phenolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tanaostigmodes ringueleti; Tanaostigmodes mecanga; Gall; Insect-plant interaction; Phenol.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000300013
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Simple models for the continuous aerobic biodegradation of phenol in a packed bed reactor BABT
Gerrard,Andrew Mark; Páca Júnior,Jan; Kostecková,Alena; Páca,Jan; Stiborová,Marie; Soccol,Carlos Ricardo.
This paper proposes the use of a preliminary, phenol removal step to reduce peak loads arriving at a conventional effluent plant. A packed bed reactor (PBR) using polyurethane foam, porous glass and also cocoa fibres as the inert support material was used. Experiments have been carried out where the flow-rates, plus inlet and outlet phenol concentrations were measured. A simple, plug-flow model is proposed to represent the results. Zero, first order, Monod and inhibited kinetics rate equations were evaluated. It was found that the Monod model gave the best fit to the experimental data and allowed linear graphs to be plotted. The Monod saturation constant, K, is approximately 50 g m-3, and ka is around 900 s-1.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Phenol; Aerobic reactor; Models.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500018
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Spectrophotometric determination of phenol in the presence of congeners by multivariated calibration Anais da ABC (AABC)
SOUZA,KELY VIVIANE DE; PERALTA-ZAMORA,PATRICIO.
The generation of poly-hydroxilated transient species during the photochemical treatment of phenol usually impedes the spectrophotmetric monitoring of its degradation process. Frequently, the appearance of compounds such as pyrocatechol, hydroquinone and benzoquinone produces serious spectral interference, which hinder the use of the classical univariate calibration process. In this work, the use of multivariate calibration is proposed to permit the spectrophotometric determination of phenol in the presence of these intermediates. Using 20 synthetic mixtures containing phenol and the interferents, a calibration model was developed by using a partial least square regression process (PLSR) and processing the absorbance signal between 180 and 300 nm. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phenol; Spectrophotometric determination; Multivariated calibration.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652001000400005
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Superficial distribution of aromatic compounds and geomicrobiology of sediments from Suruí Mangrove, Guanabara Bay, RJ, Brazil Anais da ABC (AABC)
Fontana,Luiz F.; Silva,Frederico S. da; Figueiredo,Natália G. de; Brum,Daniel M.; Netto,Annibal D. Pereira; Gigueiredo Junior,Alberto G. de; Crapez,Mirian A.C..
The distribution of selected aromatic compounds and microbiology were assessed in superficial sediments from Suruí Mangrove, Guanabara Bay. Samples were collected at 23 stations, and particle size, organic matter, aromatic compounds, microbiology activity, biopolymers, and topography were determined. The concentration of aromatic compounds was distributed in patches over the entire mangrove, and their highest total concentration was determinated in the mangrove's central area. Particle size differed from most mangroves in that Suruí Mangrove has chernies on the edges and in front of the mangrove, and sand across the whole surface, which hampers the relationship between particle size and hydrocarbons. An average @ 10% p/p of organic matter was obtained, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiologic activity; Guanabara Bay; Biopolymers; Phenol; Aromatic Polycyclic Hydrocarbons and Monoaromatics.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400022
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